Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.Application
Western blot analysis of MYL9 phosphorylation expression in untreated (A) and serum-starved (B) 293 whole cell lysates.| Product Name | p-MYL9 Antibody (Ser 19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 19 phosphorylated MYL9, nonsarcomeric and smooth muscle isoforms of m, r and h origin by WB, IP, IF and IHC(P); may cross-react with correspondingly phosphorylated MRLC2 and MRCL3 of human origin and Mylc2b of mouse and rat |
| Modification | Phosphoralated |
| Modification Notes | Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of myosin have been cloned: smooth muscle myosins, striated muscle myosins and non-muscle myosins. Myosin light chain 9, which is encoded by MYL9, is one of the numerous regulatory myosin light chains. Regulatory myosin light chains, also known as MLCs, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the Actin-activated myosin ATPase activity, thereby regulating the contractile activity. Myosin light chain is also located in striated skeletal muscle, where its function remains undefined. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 19 phosphorylated MYL9 nonsarcomeric and smooth muscle isoforms of m r and h origin by WB IP IF and IHC(P) may cross-react with correspondingly phosphorylated MRLC2 and MRCL3 of human origin and Mylc2b of mouse and rat |
|---|---|
| Tissue Specificity | recommended for detection of Ser 19 phosphorylated MYL9, nonsarcomeric and smooth muscle isoforms of m, r and h origin by WB, IP, IF and IHC(P); may cross-react with correspondingly phosphorylated MRLC2 and MRCL3 of human origin and Mylc2b of mouse and rat origin |
| Entrez Gene | 10398 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.
Application
Western blot analysis of MYL9 phosphorylation expression in untreated (A) and serum-starved (B) 293 whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
