Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Product Name | Scrib Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1331-1630 (deletion 1444-1504) mapping at the C-terminus of Scrib of human origin |
| Modification Notes | Drosophila melanogaster genes, which are categorized based on the type of protein for which they encode, represent six major classifications, including intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing and chromatin associated) and other functional proteins. Morphogenesis and cell differentiation in Drosophila requires accurate control of cell division. Discs large (Dlg), Scribble (Scrib) and Lethal giant larvae (Lgl) tumor suppressor proteins regulate multiple aspects of neuroblast asymmetric cell division. Dlg/Scrib/Lgl proteins show apical cortical enrichment at prophase/metaphase and have a uniform cortical distribution. Mutations in the genes encoding multi-PDZ (PSD-95, Discs-large and ZO-1) and the leucine-rich-repeat protein Scrib cause aberrant cell shapes and the loss of monolayer organization of embryonic epithelia. The human homolog, hScrib, is intracellularly localized to the vertebrate tight junction, which functions to correctly place adherens junctions. The PDZ domains of Scrib are predicted to bind to the consensus S/TXV at the C-terminus of proteins. PDZ domain proteins have been implicated at several different sites of the protein trafficking pathway, suggesting that Scrib is required for the localization of several epithelial determinants. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Scrib of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1331-1630 (deletion 1444-1504) mapping at the C-terminus of Scrib of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1331-1630 (deletion 1444-1504) mapping at the C-terminus of Scrib of human origin |
| Entrez Gene | 23513 |
|---|
Application
Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.| Application Notes | recommended for detection of Scrib of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
