Application
Western blot analysis of p38β expression in non-transfected: (A) and human p38β transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of bone marrow poietic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Antibodies tested include p38α (N-20): (A,B) and p38α (C-20): (C-F).| Product Name | p38α Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of p38α of mouse origin |
| Modification Notes | MAP (mitogen-activated protein) kinases play a significant role in many biological processes, including cell adhesion and spreading, cell differentiation and apoptosis. p38å, p38∫ and p38©, also known as MAPK14, MAPK11 and MAPK12, respectively, each contain one protein kinase domain and belong to the MAP kinase family. Expressed in different areas throughout the body with common expression patterns in heart, p38 proteins use magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins. Via their catalytic activity, p38å, p38∫ and p38© are involved in a variety of events throughout the cell, including signal transduction pathways, cytokine production and cell proliferation and differentiation. The p38 proteins are subject to phosphoryation on Thr and Tyr residues, an event which is thought to activate the phosphorylated protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p38α and, to a lesser extent, p38β of mouse, rat and human origin of WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of p38&alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of p38α of mouse origin |
| Entrez Gene | 1432 |
|---|
Application
Western blot analysis of p38β expression in non-transfected: (A) and human p38β transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of bone marrow poietic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Antibodies tested include p38α (N-20): (A,B) and p38α (C-20): (C-F).| Application Notes | recommended for detection of p38α and, to a lesser extent, p38β of mouse, rat and human origin of WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
