Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of Trk expression in PC-12 whole cell lysate.Application
Western blot analysis of Trk expression in retinoic acid-induced EC P19 cells at IgG concentrations of 5.0 µg/ml (A) and 1.0 µg/ml (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin tissue showing membrane staining.| Product Name | Trk Antibody (C-14) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the C-terminus of Trk of human origin |
| Modification Notes | The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on Tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for neurotrophin-3 (NT-3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Trk A, Trk B and Trk C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the C-terminus of Trk of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the C-terminus of Trk of human origin |
| Entrez Gene | 4914 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of Trk expression in PC-12 whole cell lysate.
Application
Western blot analysis of Trk expression in retinoic acid-induced EC P19 cells at IgG concentrations of 5.0 µg/ml (A) and 1.0 µg/ml (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin tissue showing membrane staining.| Application Notes | recommended for detection of Trk A, Trk B and Trk C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
