Application
Western blot analysis of human recombinant IL-10.| Product Name | IL-10 Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 19-178 representing mature IL-10 of human origin |
| Modification Notes | Interleukin-10, or IL-10, is a 178 amino acid protein that is primarily secreted by TH2 clones. IL-10 has dual functions, the first of which is the suppression of cytokine production by TH1 clones responding to antigen presented by monocyte and macrophage antigen presenting cells (APCs). The second function consists of the inhibition of response of cytokine targeted cells, possibly by the downregulation of CD25 (the IL-2 receptor) on macrophages and B lymphocytes. Human and murine IL-10 exhibit 81% sequence identity at the amino acid level and share 73% identity at the cDNA level. Both human and murine IL-10 are acid-liable and exist as non-covalently-linked homodimers in solution. IL-10 exerts its biological activity through the IL-10 receptor (IL-10R), a glycoprotein whose expression can be induced in cultured macrophages and fibroblasts by lipopolysaccharide (LPS) stimulation. IL-10 expression has been shown to be elevated in HIV-1 infected individuals and has been implicated in the progression of the disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of IL-10 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 19-178 representing mature IL-10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 19-178 representing mature IL-10 of human origin |
| Entrez Gene | 3586 |
|---|
Application
Western blot analysis of human recombinant IL-10.| Application Notes | recommended for detection of IL-10 of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
