Application
Western blot analysis of Trk expression in SK-N-MC (A), SK-N-SH (B), IMR-32 (C) and U-87 (D) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovarian cancer tissue showing membrane and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Note red immunofluorescence staining of Trk B (A) and Trk C (B) and green TUNEL staining marking apoptotic cells. Kindly provided by Hair Research Group, Humboldt University, Berlin.Application
Western blot analysis of Trk expression in rat brain extract.| Product Name | Trk Antibody (C-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Trk of porcine origin |
| Modification Notes | The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on Tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for neurotrophin-3 (NT-3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Trk A, Trk B and Trk C of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Trk of porcine origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Trk of porcine origin |
| Entrez Gene | 4916 |
|---|
Application
Western blot analysis of Trk expression in SK-N-MC (A), SK-N-SH (B), IMR-32 (C) and U-87 (D) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovarian cancer tissue showing membrane and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Note red immunofluorescence staining of Trk B (A) and Trk C (B) and green TUNEL staining marking apoptotic cells. Kindly provided by Hair Research Group, Humboldt University, Berlin.
Application
Western blot analysis of Trk expression in rat brain extract.| Application Notes | recommended for detection of Trk A, Trk B and Trk C of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
