Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse spleen tissue showing extracellular localization.Application
Western blot analysis of human recombinant TNFβ (A,B).| Product Name | TNFβ Antibody (H-171) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 35-205 representing mature tumor necrosis factor beta (TNFβ) of human origin |
| Modification Notes | Tumor necrosis factor b (TNFb), also known as lymphotoxin, is a pleiotropic cytokine. TNFa, also known as cachectin, is a smaller cytokine that binds to the same receptors, producing a vast array of effects similar to those of TNFb. TNFb and TNFa share 30% amino acid homology and have similar biological activities. TNFb is produced by activated lymphocytes, including CD4(+) T helper cell type 1 lymphocytes, CD8(+) lymphocytes and certain B lymphoblastoid cell lines. TNFa is produced by several different cell types, including lymphocytes, neutrophils and macrophages. TNFa and TNFb can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth. Target tumor cells must express TNF receptors 1 and 2 to be killed, with the p55 receptor mediating the cytotoxic response. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TNFβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 35-205 representing mature tumor necrosis factor beta (TNF&beta ) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 35-205 representing mature tumor necrosis factor beta (TNFβ) of human origin |
| Entrez Gene | 4049 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse spleen tissue showing extracellular localization.
Application
Western blot analysis of human recombinant TNFβ (A,B).| Application Notes | recommended for detection of TNFβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
