Application
Western blot analysis of AMPKβ2 expression in non-transfected: (A) and mouse AMPKβ2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AMPKβ2 expression in non-transfected: (A) and human AMPKβ2 transfected: (B) 293T whole cell lysate.Application
Western blot analysis of AMPKβ2 expression in rat skeletal muscle tissue extract.| Product Name | AMPKβ2 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-75 of AMPKβ2 of human origin |
| Modification Notes | AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic å subunit and regulatory ∫ and © subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKå1 and AMPKå2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPK∫1 encodes a 271 amino acid protein and human AMPK∫2 encodes a 272 amino acid protein. The human AMPK©1 gene encodes a 331 amino acid protein. Human AMPK©2 and AMPK©3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AMPKβ2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-75 of AMPK&beta 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-75 of AMPKβ2 of human origin |
| Entrez Gene | 5565 |
|---|
Application
Western blot analysis of AMPKβ2 expression in non-transfected: (A) and mouse AMPKβ2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AMPKβ2 expression in non-transfected: (A) and human AMPKβ2 transfected: (B) 293T whole cell lysate.
Application
Western blot analysis of AMPKβ2 expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of AMPKβ2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
