Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of glandular cells.Application
Antibodies tested include HSP 47 (G-12): (A,B) and HSP 47 (H-300): (C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human fetal kidney showing cytoplasmic staining.| Product Name | HSP 47 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 129-300 of HSP 47 of human origin |
| Modification Notes | Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. HSP 47, also known as collagen 1, serpinh1, collagen-binding protein 1 (CBP1) and gp46, is expressed in smooth muscle cells, specifically in the interstitial space between tubules, vascular smooth muscle and medullary rays. It is expressed constitutively in cells that synthesize collagen and is involved in Collagen Type I biosynthesis. HSP 47 plays a vital role in folding and assembling collagen. A procollagen-specific molecular chaperone, HSP 47 resides in the endoplasmic reticulum of procollagen-producing cells and is essential for secretion of procollagen from cells. After insult, it acts as a stress response molecule to sequester abnormal procollagen. HSP 47 synthesis is induced by TGF? and IL-1?. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HSP 47 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 129-300 of HSP 47 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 129-300 of HSP 47 of human origin |
| Entrez Gene | 871 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of glandular cells.
Application
Antibodies tested include HSP 47 (G-12): (A,B) and HSP 47 (H-300): (C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human fetal kidney showing cytoplasmic staining.| Application Notes | recommended for detection of HSP 47 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
