Application
Western blot analysis of Laminin β-2 expression in non-transfected: (A) and mouse Laminin β-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Laminin β-2 expression in HeLa (A), HT-1080 (B), LNCap (C) and DU 145 (D) whole cell lysates.Application
Western blot analysis of Laminin β-2 expression in HT-1080 (A) and HeLa (B) whole cell lysates.| Product Name | Laminin β-2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1549-1798 mapping at the C-terminus of Laminin β-2 of human origin |
| Modification Notes | The laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated å, ∫ and ©. A major component of the basal lamina, laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues. During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells. The ∫-2 Laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of a vessel, a phenomenon which has been termed extra-vascular migratory metastasis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Laminin β-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1549-1798 mapping at the C-terminus of Laminin &beta -2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1549-1798 mapping at the C-terminus of Laminin β-2 of human origin |
| Entrez Gene | 3913 |
|---|
Application
Western blot analysis of Laminin β-2 expression in non-transfected: (A) and mouse Laminin β-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Laminin β-2 expression in HeLa (A), HT-1080 (B), LNCap (C) and DU 145 (D) whole cell lysates.
Application
Western blot analysis of Laminin β-2 expression in HT-1080 (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of Laminin β-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
