Application
Antibodies tested include p-ZAP-70 (Tyr 319)-R: (A,B,C) and ZAP-70 (LR): (D,E,F).Application
Western blot analysis of ZAP-70 expression in non-transfected: (A) and human ZAP-70 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ZAP-70 expression in non-transfected 293T: (A), mouse ZAP-70 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of ZAP-70 expression in CCRF-CEM (A) and BYDP (B) whole cell lysates.| Product Name | ZAP-70 Antibody (LR) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the “linker” region of ZAP-70 of human origin |
| Modification Notes | The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR) which is a multisubunit complex assembled from at least six different genes. The TCR subunits include the Ti å and ß chains, the CD3 ©, ∂ and e chains and a Ω-containing homodimer or heterodimer. The disulfide-linked Ti å-ß heterodimer is responsible for antigen recognition, but the short 5 amino acid cytoplasmic domains of Ti å and ß are unlikely to be sufficient to couple to intracellular signaling pathways. In contrast, the structured features of the CD3 and Ω subunits suggest a role in signal transduction. Of these, the Ω chain, which is expressed as either a homodimer or heterodimer, has a short extracellular domain of only 9 amino acids, but a larger 113 amino acid cytoplasmic domain. A tyrosine phosphoprotein, ZAP-70, has been identified that associates with z and undergoes tyrosine phosphorylation following TCR stimulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| produced using a polyhistidine fusion protein immunogen: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the “linker” region of ZAP-70 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within the “linker” region of ZAP-70 of human origin |
| Entrez Gene | 7535 |
|---|
Application
Antibodies tested include p-ZAP-70 (Tyr 319)-R: (A,B,C) and ZAP-70 (LR): (D,E,F).
Application
Western blot analysis of ZAP-70 expression in non-transfected: (A) and human ZAP-70 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ZAP-70 expression in non-transfected 293T: (A), mouse ZAP-70 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of ZAP-70 expression in CCRF-CEM (A) and BYDP (B) whole cell lysates.| Application Notes | produced using a polyhistidine fusion protein immunogen: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
