Application
Western blot analysis of C/EBP β expression in non-transfected 293T: (A), human C/EBP β transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing nuclear localization.Application
Western blot analysis of human recombinant C/EBP β fusion protein.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear staining of select cells.| Product Name | C/EBP β Antibody (Δ 198) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 199-345 of C/EBP β of human origin |
| Modification Notes | CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP ∫ is a member of the C/EBP transcription factor family. The C/EBP ∫ gene encodes several isoforms containing truncated transcription activation domains due to the alternative translational initiation at multipe AUG start sites. Initiation of translation at the in-frame AUGs forms 4 C/EBP ∫ isoforms. C/EBP ∫ is also known as interleukin 6-dependent DNA-binding protein (IL6DBP), liver activator protein (LAP) or liver-enriched transcriptional activator protein transcription factor 5 (TCF5). C/EBP ∫ contributes to the regulation of the acute phase response in hepatocytes. Stat3 has an important function in IL-6-mediated transcription of the C/EBP ∫ gene that has direct implication for acute phase response in liver cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of C/EBP β, C/EBP α, C/EBP δ and C/EBP ε of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 199-345 of C/EBP &beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 199-345 of C/EBP β of human origin |
| Entrez Gene | 1051 |
|---|
Application
Western blot analysis of C/EBP β expression in non-transfected 293T: (A), human C/EBP β transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed MCF7 cells showing nuclear localization.
Application
Western blot analysis of human recombinant C/EBP β fusion protein.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue showing nuclear staining of select cells.| Application Notes | recommended for detection of C/EBP β, C/EBP α, C/EBP δ and C/EBP ε of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
