Application
Western blot analysis of phosphorylated IKKα expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic staining.| Product Name | p-IKKα Antibody (Thr 23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Thr 23 of IKKα of human origin |
| Modification | Phosphoralated |
| Modification Notes | The transcription factor NFkB is retained in the cytoplasm in an inactive form by the inhibitory protein IkB. Activation of NFkB requires that IkB be phosphorylated on specific Serine residues, which results in targeted degradation of IkB. IkB kinase a (IKKa) interacts with IkB-a and specifically phosphorylates IkB-a on the sites that trigger its degradation, Serines 32 and 36. The functional IKK complex contains three subunits, designated IKKa, IKKb and IKKg (also designated NEMO); each appears to make essential contributions to IkB phosphorylation. NFkB inducing kinase (NIK) phosphorylates IKKa at Serine 176. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Thr 23 phosphorylated IKKα of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Thr 23 of IKK&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Thr 23 of IKKα of human origin |
| Entrez Gene | 1147 |
|---|
Application
Western blot analysis of phosphorylated IKKα expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Thr 23 phosphorylated IKKα of mouse, rat and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
