Application
Western blot analysis of Importin-11 expression in non-transfected: (A) and human Importin-11 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Importin-11 expression in HeLa whole cell lysate in the absence (A) and the presence (B) of immunizing peptide.| Product Name | Importin-11 Antibody (N-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Importin-11 of mouse, rat and human origin by WB and IP; also reactive with additional species, including bovine and canine |
| Modification Notes | The Importin complex consists of Importin-å and Importin-∫ proteins which assist in the transport of arginine- or serine-rich (SR) peptides across the nucleus. Importin-11, also known as IPO11 or RanBP11, is a 975 amino acid protein that contains one importin N-terminal domain and 15 HEAT repeats and belongs to the Importin-∫ family. Localized to both the nucleus and the cytoplasm, Importin-11 interacts with UBE2E3 and plays a role in nuclear protein import, specifically functioning as a nuclear transport receptor that mediates the docking of the Importin complex to the nuclear pore complex (NPC). The gene encoding Importin-11 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Importin-11 of mouse rat and human origin by WB and IP also reactive with additional species including bovine and canine |
|---|---|
| Tissue Specificity | recommended for detection of Importin-11 of mouse, rat and human origin by WB and IP; also reactive with additional species, including bovine and canine |
| Entrez Gene | 51194 |
|---|
Application
Western blot analysis of Importin-11 expression in non-transfected: (A) and human Importin-11 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Importin-11 expression in HeLa whole cell lysate in the absence (A) and the presence (B) of immunizing peptide.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
