Application
Western blot analysis of B7RP-1 expression in non-transfected: (A) and human B7RP-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of B7RP-1 expression in Daudi (A) and human PBL (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Ramos cells showing membrane localization.| Product Name | B7RP-1 Antibody (FL-302) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-302 representing full length B7RP-1 of human origin |
| Modification Notes | T cell costimulatory molecule, inducible costimulator (ICOS)/B7-related protein-1 (B7RP-1, B7-H2, GL50, ICOS-L) is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. B7RP-1 interactions play an essential role in T cell dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. B7RP-1 protein is present in myeloid leukocytes and by Northern blot there are 2.4, 3.0 and 7.0 kb transcripts in brain, heart, kidney and liver, with lower expression in colon and thymus and a 1.1 kb transcript in leukocytes. Tumor necrosis factor å (TNFå), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4) enhance B7RP-1 expression. LPS induced up regulation of B7RP-1 is dependent on the MyD88 dependent signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of B7RP-1 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-302 representing full length B7RP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-302 representing full length B7RP-1 of human origin |
| Entrez Gene | 23308 |
|---|
Application
Western blot analysis of B7RP-1 expression in non-transfected: (A) and human B7RP-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of B7RP-1 expression in Daudi (A) and human PBL (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Ramos cells showing membrane localization.| Application Notes | recommended for detection of B7RP-1 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
