Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane staining of glandular cells.Application
Western blot analysis of α N-catenin expression in non-transfected 293T: (A), human α N-catenin transfected 293T: (B) and T98G (C) whole cell lysates.Application
Western blot analysis of α E-catenin expression in non-transfected 293T: (A), mouse α E-catenin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of α N-catenin expression in non-transfected 293T: (A), human α N-catenin transfected 293T: (B) and HeLa (C) whole cell lysate.Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane (cell-cell adhesion) staining.Application
Western blot analysis of α E-catenin expression in c4 (A) and WEHI-231 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast showing membrane and cytoplasmic staining.Application
Western blot analysis of α E-catenin expression in HeLa (A) and A-431 (B) whole cell lysates.| Product Name | α E-catenin Antibody (H-297) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 610-906 mapping at the C-terminus of α E-catenin of human origin |
| Modification Notes | å E-catenin (also designated å-catenin; cadherin-associated protein, å1, 102 kDa; and CAP102) plays a role in E-cadherin mediated cell-cell adhesion by linking E-cadherin to the cytoskeleton via ∫- or ©-catenin and Actin. å E-catenin connects cell-density-dependent adherens junctions with the developmental hedgehog pathway and may provide a negative feedback loop controlling the size of developing cerebral cortex. It is abundant in neuroepithelial precursor cells in the developing cortical ventricular zone of the brain, with reduced expression in the cortical plate. å E-catenin-vinculin interactions play a role in the assembly of the apical junction complex in epithelia. Catenins generally are thought to work as connectors that anchor E-cadherin to the cytoskeletal Actin bundle through the cadherin cytoplasmic domain. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of α E-catenin and α N-catenin of mouse, rat, human, zebrafish and Drosophila origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 610-906 mapping at the C-terminus of &alpha E-catenin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 610-906 mapping at the C-terminus of α E-catenin of human origin |
| Entrez Gene | 1495 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing membrane staining of glandular cells.
Application
Western blot analysis of α N-catenin expression in non-transfected 293T: (A), human α N-catenin transfected 293T: (B) and T98G (C) whole cell lysates.
Application
Western blot analysis of α E-catenin expression in non-transfected 293T: (A), mouse α E-catenin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of α N-catenin expression in non-transfected 293T: (A), human α N-catenin transfected 293T: (B) and HeLa (C) whole cell lysate.
Application
Immunofluorescence staining of normal mouse heart frozen section showing membrane (cell-cell adhesion) staining.
Application
Western blot analysis of α E-catenin expression in c4 (A) and WEHI-231 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast showing membrane and cytoplasmic staining.
Application
Western blot analysis of α E-catenin expression in HeLa (A) and A-431 (B) whole cell lysates.| Application Notes | recommended for detection of α E-catenin and α N-catenin of mouse, rat, human, zebrafish and Drosophila origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
