| Product Name | α-mannosidase Antibody (Y-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 732-810 mapping at the C-terminus of α-mannosidase of Saccharomyces cerevisiae origin |
| Modification Notes | Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. The proper functioning of eukaryotic organelles is largely dependent on the specific packaging of cargo proteins within transient delivery vesicles (1). The cytoplasm to vacuole targeting (Cvt) pathway is an autophagy-related trafficking pathway whose cargo proteins, aminopeptidase I and å-mannosidase, are selectively transported from the cytoplasm to the lysosome-like vacuole in yeast (1-3). In the budding yeast Saccharomyces cerevisiae, å-mannosidase, also known as AMP Deaminase or Ams1, is a marker enzyme of the vacuolar membrane (4,5). å-mannosidase has no typical signal sequence, but is located on the inner surface of the vacuolar membrane (4). The enzyme is synthesized as a polypeptide and is converted to a shorter polypeptide by the vacuolar processing protease, proteinase A (4). å-mannosidase forms an oligomer in the cytoplasm, however its mechanism of vacuolar delivery has not been established (5). Vacuolar localization of å-mannosidase is blocked in mutants that are defective in the Cvt and autophagy pathways (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of α-mannosidase of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 732-810 mapping at the C-terminus of &alpha -mannosidase of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 732-810 mapping at the C-terminus of α-mannosidase of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of α-mannosidase of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
