Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of ERK 3 expression in non-transfected: (A) and human ERK 3 transfected: (B) 293T whole cell lysates.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .| Product Name | ERK 3 Antibody (D-23) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping subdomain XI of ERK 3 of rat origin |
| Modification Notes | Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl-2 and caspase-3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK 3 gene maps to chromosome 15q21.2 and encodes a 721 amino acid protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of ERK 3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping subdomain XI of ERK 3 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping subdomain XI of ERK 3 of rat origin |
| Entrez Gene | 5597 |
|---|
Application
Intracellular FCM analysis of fixed and permeabilized A-431 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of ERK 3 expression in non-transfected: (A) and human ERK 3 transfected: (B) 293T whole cell lysates.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .| Application Notes | recommended for detection of ERK 3 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
