Application
Western blot analysis of VDR expression in non-transfected: (A) and mouse VDR transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of VDR expression in HL-60 whole cell lysate (A) and NIH/3T3 (B) and MCF7 (C) nuclear extracts.Application
Western blot analysis of VDR expression in KNRK whole cell lysate.| Product Name | VDR Antibody (C-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of VDR of rat origin |
| Modification Notes | The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above mentioned cellular responses, 1,25-(OH) 2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of VDR of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of VDR of rat origin |
| Entrez Gene | 7421 |
|---|
Application
Western blot analysis of VDR expression in non-transfected: (A) and mouse VDR transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of VDR expression in HL-60 whole cell lysate (A) and NIH/3T3 (B) and MCF7 (C) nuclear extracts.
Application
Western blot analysis of VDR expression in KNRK whole cell lysate.| Application Notes | recommended for detection of VDR of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
