Application
Indirect FCM analysis of BJAB cells stained with CD10 (H-321), followed by PerCP-conjugated donkey anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Indirect FCM analysis of BJAB cells stained with CD10 (H-321), followed by PE-conjugated donkey anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of CD10 expression in non-transfected: (A) and mouse CD10 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD10 expression in non-transfected: (A) and human CD10 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed LNCaP cells showing membrane staining.Application
Western blot analysis of CD10 expression in LNCaP whole cell lysate.| Product Name | CD10 Antibody (H-321) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 230-550 mapping within an internal region of CD10 of human origin |
| Modification Notes | CD10, also called the common acute lymphoblastic leukemia antigen (CALLA) and neutral endopeptidase (NEP), is a type II integral membrane glycoprotein. CD10 acts as a zinc metalloprotease that cleaves a variety of biologically active peptides including Angiotensins I and II. It is expressed on early B and T lymphoid precursors, B blasts, some granulocytes, bone marrow stromal cells and certain epithelial cells including some tumor cell lines. CD10 is used as a marker of common acute lymphocytic leukemias and some lymphomas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of CD10 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 230-550 mapping within an internal region of CD10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 230-550 mapping within an internal region of CD10 of human origin |
| Entrez Gene | 4311 |
|---|
Application
Indirect FCM analysis of BJAB cells stained with CD10 (H-321), followed by PerCP-conjugated donkey anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Indirect FCM analysis of BJAB cells stained with CD10 (H-321), followed by PE-conjugated donkey anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of CD10 expression in non-transfected: (A) and mouse CD10 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD10 expression in non-transfected: (A) and human CD10 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed LNCaP cells showing membrane staining.
Application
Western blot analysis of CD10 expression in LNCaP whole cell lysate.| Application Notes | recommended for detection of CD10 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
