Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts and Leydig cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic and nuclear staining of Islets of Langerhans and glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of HSP 70-2 expression in non-transfected 293T: (A), human HSP 70-2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of HSP 70-2 expression in non-transfected 293T: (A), human HSP 70-2 transfected 293T: (B) and HUV-EC-C (C) whole cell lysates.Application
Western blot analysis of HSP 70/HSC 70 expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of HSP 70/ HSC 70 expression in non-transfected: (A) and human HSC 70 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HSP 70/HSC 70 expression in 293 (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of HSP 70/HSC 70 expression in HEK293 whole cell lysate.Application
Western blot analysis of HSP 70 expression in HeLa (A), HeLa heat shock treated (B), NIH/3T3 (C) and NIH/3T3 heat shock treated (D) whole cell lysates.Application
Western blot analysis of HSP 70/HSC 70 expression in HSP treated HeLa whole cell lysate.| Product Name | HSP 70/HSC 70 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 342-641 mapping at the C-terminus of Hsp 70 of human origin |
| Modification Notes | The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. These proteins serve a variety of roles: they act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. All members of the family, except HSP 70, are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery, and are found in both the cytosol and nucleus of mammalian cells. Both HSP 70 and HSC 70 are involved in the chaperoning of nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HSP 70 and HSC 70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 342-641 mapping at the C-terminus of Hsp 70 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 342-641 mapping at the C-terminus of Hsp 70 of human origin |
| Entrez Gene | 3303 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts and Leydig cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic and nuclear staining of Islets of Langerhans and glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of HSP 70-2 expression in non-transfected 293T: (A), human HSP 70-2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of HSP 70-2 expression in non-transfected 293T: (A), human HSP 70-2 transfected 293T: (B) and HUV-EC-C (C) whole cell lysates.
Application
Western blot analysis of HSP 70/HSC 70 expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of HSP 70/ HSC 70 expression in non-transfected: (A) and human HSC 70 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HSP 70/HSC 70 expression in 293 (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of HSP 70/HSC 70 expression in HEK293 whole cell lysate.
Application
Western blot analysis of HSP 70 expression in HeLa (A), HeLa heat shock treated (B), NIH/3T3 (C) and NIH/3T3 heat shock treated (D) whole cell lysates.
Application
Western blot analysis of HSP 70/HSC 70 expression in HSP treated HeLa whole cell lysate.| Application Notes | recommended for detection of HSP 70 and HSC 70 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
