Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of decidual cells.Application
Western blot analysis of IL-8RA expression in non-transfected 293: (A), mouse IL-8RA transfected 293: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of IL-8RA/B expression in RAW 264.7 (A), CTLL-2 (B), mouse PBL (C) and J774.A1 (D) whole cell lysates.Application
Western blot analysis of IL-8RA expression in non-transfected: (A) and human IL-8RA transfected: (B) 293T whole cell lysates.| Product Name | IL-8RA/B Antibody (H-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 141-240 mapping within an internal region of IL-8RA of human origin |
| Modification Notes | IL-8 has been shown to function as a potent neutrophil chemostatic and activating peptide and is an important mediator of inflammatory diseases. Two distinct human IL-8 receptors, designated IL-8RA and IL-8RB have been characterized. Both are expressed at a high level on neutrophils and to a lesser extent on monocytes and myeloid cell lines. In addition, the IL-8RA subunit is expressed in T cells such as the Jurkat cell line. Both IL-8Rs are members of the seven-transmembrane domain rhodopsin superfamily of receptors and as such, couple G proteins for signal transduction. The two receptors share 77% amino acid identity. IL-8RA exhibits high-affinity binding for IL-8 and low-affinity MGSA binding, whereas IL-8RB has high-affinity binding for both IL-8 and MGSA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IL-8RA and B of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 141-240 mapping within an internal region of IL-8RA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 141-240 mapping within an internal region of IL-8RA of human origin |
| Entrez Gene | 3577 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing cytoplasmic staining of decidual cells.
Application
Western blot analysis of IL-8RA expression in non-transfected 293: (A), mouse IL-8RA transfected 293: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of IL-8RA/B expression in RAW 264.7 (A), CTLL-2 (B), mouse PBL (C) and J774.A1 (D) whole cell lysates.
Application
Western blot analysis of IL-8RA expression in non-transfected: (A) and human IL-8RA transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IL-8RA and B of mouse, rat and human origin by WB, IP, IF,IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
