Application
Western blot analysis of Pygopus 1 expression in JAR whole cell lysate.| Product Name | Pygopus 1 Antibody (H-162) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 65-221 mapping within an internal region of Pygopus 1 of human origin |
| Modification Notes | Pygopus 1, also known as PYGO1, is a 419 amino acid protein that localizes to the nucleus and contains one PHD-type zinc finger. Interacting with Bcl-9, Pygopus 1 is thought to be involved in signal transduction events related to the Wnt pathway. The gene encoding Pygopus 1 maps to human chromosome 15, which encodes over 700 genes and comprises nearly 3% of the human genome. Angelman and Prader-Willi syndromes are associated with loss of function or deletion of genes in the 15q11-q13 region. In the case of Angelman syndrome, this loss is due to inactivity of the maternal 15q11-q13 encoded UBE3A gene in the brain by either chromosomal deletion or mutation. In cases of Prader-Willi syndrome, there is a partial or complete deletion of this region from the paternal copy of chromosome 15. Tay-Sachs disease is a lethal disorder associated with mutations of the HEXA gene, which is encoded by chromosome 15. Marfan syndrome is associated with chromosome 15 through the FBN1 gene. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Pygopus 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 65-221 mapping within an internal region of Pygopus 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 65-221 mapping within an internal region of Pygopus 1 of human origin |
| Entrez Gene | 26108 |
|---|
Application
Western blot analysis of Pygopus 1 expression in JAR whole cell lysate.| Application Notes | recommended for detection of Pygopus 1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
