Application
Western blot analysis of hnRNP C1/C2 expression in non-transfected: (A) and human hnRNP C1/C2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of hnRNP C1/C2 expression in non-transfected 293T: (A) and human hnRNP C1/C2 transfected 293T: (B) whole cell lysates and HeLa nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human endometrium tissue showing nuclear staining of endometrial stroma, myometrum and glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue (B) showing nuclear localization.Application
Western blot analysis of hnRNP C1/C2 expression in Jurkat (A) and HeLa (B) nuclear extracts.| Product Name | hnRNP C1/C2 Antibody (H-105) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 86-190 of hnRNP C1/C2 of human origin |
| Modification Notes | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to pre-mRNA processing and transport, and also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP complexes are the major constituents of the spliceosome and, in particular, the hnRNP A1 protein is one of the major pre-mRNA/mRNA binding proteins and also one of the most abundant proteins in the nucleus. hnRNP A1 and A2/B1 regulate the processing of pre-mRNA by directly antagonizing the association of various splicing factors and by influencing the splice site selection on pre-mRNA. The majority of hnRNP proteins components are localized to the nucleus; however, some shuttle between the nucleus and the cytoplasm. Most hnRNP proteins, in-cluding hnRNP C1 and C2, contain one or more RNA binding domains and are implicated in the processing of pre-mRNA. hnRNPs F and H are largely related factors that preferentially associate with poly(rG) regions on RNA. Isoforms of these proteins are often generated by alternative processing of the pre-mRNA and by posttranslational modifications such as phosphorylation on serines and threonines and methylation of arginines. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of hnRNP C1/C2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 86-190 of hnRNP C1/C2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 86-190 of hnRNP C1/C2 of human origin |
| Entrez Gene | 3183 |
|---|
Application
Western blot analysis of hnRNP C1/C2 expression in non-transfected: (A) and human hnRNP C1/C2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of hnRNP C1/C2 expression in non-transfected 293T: (A) and human hnRNP C1/C2 transfected 293T: (B) whole cell lysates and HeLa nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human endometrium tissue showing nuclear staining of endometrial stroma, myometrum and glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells (A) and immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse liver tissue (B) showing nuclear localization.
Application
Western blot analysis of hnRNP C1/C2 expression in Jurkat (A) and HeLa (B) nuclear extracts.| Application Notes | recommended for detection of hnRNP C1/C2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
