Application
Western blot analysis of FEN-1 expression in non-transfected 293T: (A), mouse FEN-1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of FEN-1 expression in HeLa (A) and Jurkat (B) nuclear extracts.| Product Name | FEN-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-380 of FEN-1 of human origin |
| Modification Notes | DNA replication, recombination and repair, all of which are necessary for genome stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair, they function to excise damaged DNA fragments and correct recombinational mismatches. FEN-1 (for flap endonuclease) is an endonuclease that specifically cleaves the 5’ flap structure of DNA in the process of DNA repair. FEN-1 is highly homologous to yeast RAD2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FEN-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-380 of FEN-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-380 of FEN-1 of human origin |
| Entrez Gene | 2237 |
|---|
Application
Western blot analysis of FEN-1 expression in non-transfected 293T: (A), mouse FEN-1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of FEN-1 expression in HeLa (A) and Jurkat (B) nuclear extracts.| Application Notes | recommended for detection of FEN-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
