Application
Western blot analysis of PRL-1 expression in non-transfected: (A) and human PRL-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of PRL-1 expression in non-transfected: (A) and human PRL-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PRL-1/2/3 expression in mouse brain tissue extract.Application
Western blot analysis of PRL-1 expression in non-transfected: (A), mouse PRL-1 transfected: (B) and mouse PRL-1 transfected: (C) 293T whole cell lysates.Application
Western blot analysis of PRL-1/2/3 expression in rat brain tissue extract.| Product Name | PRL-1/2/3 Antibody (FL-173) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-173 representing full length PRL-3 of human origin |
| Modification Notes | Protein tyrosine phosphatases (PTPs) play a role in regulating diverse cellular processes. They form a small class of prenylated protein phosphatases called PRL proteins characterized by a C-terminal consensus sequence for prenylation. PRL-1, also designated Protein tyrosine phosphatase type IVA protein 1 (PTP4A1), is a unique nuclear PTP that is induced in regenerating liver and mitogen-stimulated cells. It is primarily expressed in spleen, bone marrow, thymus, lymph nodes, T lymphocytes and tonsil and is overexpressed in tumor cell lines. PRL-2 (Protein tyrosine phosphatase type IVA protein 2, or PTP4A2) is ubiquitously expressed with highest levels in heart, skeletal muscle and thymus but is also overexpressed in prostate tumor tissue. PPRL-2 is stimulates progression from G1 into S phase during mitosis and promotes tumors. PRL-3, also known as Protein Tyrosine Phosphatase Type IVA, member 3 (PTP4A3) is expressed in heart and skeletal muscle as well as epithelial cells of the small intestine and associates with the cell plasma membrane. Over expression of PRL-3 inhibits angiotensin-II induced cell calcium mobilization and promotes cell growth. PRL-3 is important for colorectal cancer metastasis and may serve as a new therapeutic target for this condition. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PRL-1, PRL-2 and PRL-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-173 representing full length PRL-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-173 representing full length PRL-3 of human origin |
| Entrez Gene | 11156 |
|---|
Application
Western blot analysis of PRL-1 expression in non-transfected: (A) and human PRL-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of PRL-1 expression in non-transfected: (A) and human PRL-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PRL-1/2/3 expression in mouse brain tissue extract.
Application
Western blot analysis of PRL-1 expression in non-transfected: (A), mouse PRL-1 transfected: (B) and mouse PRL-1 transfected: (C) 293T whole cell lysates.
Application
Western blot analysis of PRL-1/2/3 expression in rat brain tissue extract.| Application Notes | recommended for detection of PRL-1, PRL-2 and PRL-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
