Application
Western blot analysis of Smad6 expression in non-transfected 293T: (A), mouse Smad6 transfected 293T: (B) and A549 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.| Product Name | Smad6 Antibody (H-150) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-190 mapping near the N-terminus of Smad6 of human origin |
| Modification Notes | Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGF∫/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGF∫ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGF∫ signaling by interfering with TGF∫-mediated phosphorylation of other Smad family members. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Smad6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-190 mapping near the N-terminus of Smad6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-190 mapping near the N-terminus of Smad6 of human origin |
| Entrez Gene | 4091 |
|---|
Application
Western blot analysis of Smad6 expression in non-transfected 293T: (A), mouse Smad6 transfected 293T: (B) and A549 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A549 cells showing cytoplasmic localization.| Application Notes | recommended for detection of Smad6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
