Application
Western blot analysis of PLAP expression in non-transfected: (A) and human PLAP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PLAP expression in mouse heart (A) and mouse placenta (B) tissue extracts.Application
Western blot analysis of PLAP expression in JAR whole cell lysate.| Product Name | Alkaline Phosphatase Antibody (H-330) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 31-330 mapping near the N-terminus of PLAP of human origin |
| Modification Notes | Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+-metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. Placental alkaline phosphatase (also known as PLAP, ALPP, PALP, placental ALP-1 or Regan isozyme) is a 530 amino acid, tissue-specific AP that is expressed in the placenta, the serum of pregnant women and ectopically expressed in various cancers, including those of the ovary and testis. PLAP may assist in guiding migratory cells and transporting specific molecules, such as fatty acids and immunoglobulins, across the plasma membrane. The three tissue-specific APs identified in human, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous and their genes are clustered on chromosome 2q. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PLAP, ALP-1, IAP, EAP and TNSALP and partially cross-reactive with other alkaline phosphatases of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-330 mapping near the N-terminus of PLAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-330 mapping near the N-terminus of PLAP of human origin |
| Entrez Gene | 250 |
|---|
Application
Western blot analysis of PLAP expression in non-transfected: (A) and human PLAP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PLAP expression in mouse heart (A) and mouse placenta (B) tissue extracts.
Application
Western blot analysis of PLAP expression in JAR whole cell lysate.| Application Notes | recommended for detection of PLAP, ALP-1, IAP, EAP and TNSALP and partially cross-reactive with other alkaline phosphatases of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
