Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Gα i-1 expression in mouse brain tissue extract.Application
Western blot analysis of Gα i-1 expression in SK-N-SH whole cell lysate.Application
Western blot analysis of Gα i-1 expression in bovine brain extract (A) and analysis of rat recombinant Gα i-1: WB (B).| Product Name | Gα i-1 Antibody (I-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping with in a highly divergent domain of Gα i-1 of rat origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter), whereas the effectors (i.e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein a, b and g subunits are encoded by at least sixteen, four and seven different genes, respectively. The a subunits bind to and hydrolyze GTP. G protein complexes expressed in different tissues contain distinct a, b and g subunits. Preferential associations between members of subunit families increase G protein functional diversity. Most interest in G proteins has been focused on their å subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gå subunits have been identified; these include Gs, Gi, Gq and Ga 12/13. The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Gå i-1, Gå i-2, Gå i-3, Gå o, Gå t1, Gå t2, Gå z and Gå gust. Of these, the three Ga i subtypes function to open atrial potassium channels. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFELISA |
| recommended for detection of Gα i-1 and, to a lesser extent, Gα i-2 and Gα i-3 of mouse, rat, human and bovine origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping with in a highly divergent domain of G&alpha i-1 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping with in a highly divergent domain of Gα i-1 of rat origin |
| Entrez Gene | 2770 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Gα i-1 expression in mouse brain tissue extract.
Application
Western blot analysis of Gα i-1 expression in SK-N-SH whole cell lysate.
Application
Western blot analysis of Gα i-1 expression in bovine brain extract (A) and analysis of rat recombinant Gα i-1: WB (B).| Application Notes | recommended for detection of Gα i-1 and, to a lesser extent, Gα i-2 and Gα i-3 of mouse, rat, human and bovine origin by WB, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
