Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic and nuclear staining of cells in glomeruli and tubules.Application
Western blot analysis of YAP expression in non-transfected: (A) and mouse YAP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of YAP expression in non-transfected: (A) and human YAP transfected: (B) 293T whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovarian cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of YAP expression in PC-3 whole cell lysate.| Product Name | YAP Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 206-330 mapping near the C-terminus of YAP of human origin |
| Modification Notes | The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 206-330 mapping near the C-terminus of YAP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 206-330 mapping near the C-terminus of YAP of human origin |
| Entrez Gene | 10413 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic and nuclear staining of cells in glomeruli and tubules.
Application
Western blot analysis of YAP expression in non-transfected: (A) and mouse YAP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of YAP expression in non-transfected: (A) and human YAP transfected: (B) 293T whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovarian cancer tissue showing nuclear and cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of YAP expression in PC-3 whole cell lysate.| Application Notes | recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
