Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatocytes.Application
Western blot analysis of Mdr expression in MES-SA/Dx5 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed MES-SA/Dx5 cells showing membrane localization.| Product Name | Mdr Antibody (H-241) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1040-1280 of Mdr-1 of human origin |
| Modification Notes | Cells selected for resistance to a single cytotoxic drug may become cross-resistant to a broad range of drugs with different structures and cellular targets. This phenomenon is called multiple drug resistance (Mdr). The Mdr proteins are members of a highly conserved superfamily of ATP-binding cassette transport proteins. Mdr functions as an energy-dependent efflux pump for structurally diverse agents ranging from ions to peptides. It is implicated in the development of the multiple drug resistance observed in human cancer cells following prolonged chemotherapy. The classic form of Mdr is associated with an increase in the Mdr protein, but not all cases of Mdr can be attributed to a rise in Mdr levels. In cell lines not expressing increased levels of Mdr, there is also an increase in the level of a protein designated Mdr-associated protein (MRP). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Mdr-1 and Mdr-3 of mouse, rat and human origin, and Mdr-2 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1040-1280 of Mdr-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1040-1280 of Mdr-1 of human origin |
| Entrez Gene | 5243 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing membrane staining of hepatocytes.
Application
Western blot analysis of Mdr expression in MES-SA/Dx5 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed MES-SA/Dx5 cells showing membrane localization.| Application Notes | recommended for detection of Mdr-1 and Mdr-3 of mouse, rat and human origin, and Mdr-2 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
