Application
Western blot analysis of DACH1 expression in HeLa (A) and Jurkat (B) whole cell lysates.| Product Name | DACH1 Antibody (H-93) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 528-620 mapping within an internal region of DACH1 of human origin |
| Modification Notes | DACH1 (Dachshund homolog 1), also known as DACH, is a 758 amino acid homolog of the Drosophila dachshund gene that encodes a nuclear factor involved in eye, leg and nervous system development. Localized to the nucleus and expressed throughout the body, DACH1 is a transcription factor that regulates the activation of a variety of genes involved in organogenesis and is crucial in proper eye formation. Through association with SMAD4 and NCOR1, DACH1 is able to inhibit the TGF-beta signaling pathway and, via its DACHbox-N domain, can bind directly to chromatin where it regulates transcription. Additionally, DACH1 can block cellular proliferation and growth of human breast cancer cells, suggesting a possible role in tumor suppression. Four isoforms of DACH1 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DACH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 528-620 mapping within an internal region of DACH1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 528-620 mapping within an internal region of DACH1 of human origin |
| Entrez Gene | 1602 |
|---|
Application
Western blot analysis of DACH1 expression in HeLa (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of DACH1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
