Application
Immunofluorescence staining of methanol-fixed HEL 92.1.7 cells showing nuclear localization.Application
Western blot analysis of PLZF expression in Hel 92.1.7 whole cell lysate.Application
Western blot analysis of PLZF expression in TF-1 whole cell lysate.| Product Name | PLZF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 101-400 mapping to an internal region of PLZF of human origin |
| Modification Notes | Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS). The expression of HIC-1 is also frequently suppressed in leu-kemia and other various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad-complex POZ (or BTB) domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ interactions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PLZF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 101-400 mapping to an internal region of PLZF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 101-400 mapping to an internal region of PLZF of human origin |
| Entrez Gene | 7704 |
|---|
Application
Immunofluorescence staining of methanol-fixed HEL 92.1.7 cells showing nuclear localization.
Application
Western blot analysis of PLZF expression in Hel 92.1.7 whole cell lysate.
Application
Western blot analysis of PLZF expression in TF-1 whole cell lysate.| Application Notes | recommended for detection of PLZF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
