Application
Western blot analysis of γ1-Adaptin expression in non-transfected 293T: (A), human γ1-Adaptin transfected 293T: (B) and HISM (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.Application
Western blot analysis of γ1-Adaptin expression in BC3H1 whole cell lysate.| Product Name | γ1-Adaptin Antibody (M-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 653-821 mapping at the C-terminus of γ1-Adaptin of mouse origin |
| Modification Notes | Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of clathrin binding adaptin proteins (g and b1 for AP-1, a and b2 for AP-2) and two smaller subunits known as AP50 and AP17. The a and b adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. a-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP180 (also designated AP-3 or F1-20) is a synapse-specific clathrin assembly protein. The protein CALM (clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in clathrin assembly. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of γ1-Adaptin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 653-821 mapping at the C-terminus of &gamma 1-Adaptin of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 653-821 mapping at the C-terminus of γ1-Adaptin of mouse origin |
| Entrez Gene | 164 |
|---|
Application
Western blot analysis of γ1-Adaptin expression in non-transfected 293T: (A), human γ1-Adaptin transfected 293T: (B) and HISM (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing membrane and cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed BC3H1 cells showing cytoplasmic localization.
Application
Western blot analysis of γ1-Adaptin expression in BC3H1 whole cell lysate.| Application Notes | recommended for detection of γ1-Adaptin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
