Application
Western blot analysis of RhoGEF p115 expression in non-transfected 293T: (A), human RhoGEF p115 transfected 293T: (B), K-562 (C) and Jurkat (D) whole cell lysates.Application
Western blot analysis of RhoGEF p115 expression in non-transfected 293T: (A), human RhoGEF p115 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of RhoGEF p115 expression in non-transfected: (A) and human RhoGEF p115 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RhoGEF p115 expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane and cytoplasmic staining of cells in white and red pulps at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of RhoGEF p115 expression in Jurkat whole cell lysate.| Product Name | RhoGEF p115/Lsc Antibody (H-165) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 748-912 mapping at the C-terminus of RhoGEF p115 of human origin |
| Modification Notes | The Ras superfamily of GTPases can be subdivided into the Ras, Rho/Rac, Sar, Rab, Arf and Ran subfamilies and controls multiple aspects of cell function, including cytoskeletal rearrangement, nuclear signaling, and cell growth. The Ras superfamily of GTPases function as regulated switches that toggle between a biologically active GTP-bound and an inactive GDP-bound form. This activation is catalyzed by guanine nucleotide exchange factors (GEFs). The Dbl-related proteins are a large family of structurally related molecules that have a common ability to catalyze GEF activity for specific members of the Ras family. Dbl-related proteins include FGD1, RhoGEF p115/Lsc, Lfc, Lbc and Brx. RhoGEF p115/Lsc, Lbc and Lfc share sequence homology and show exchange activity toward Rho family GTPases. RhoGEF p115 (the human homolog of Lsc) catalyzes GEF activity for Rho but not Rac, Cdc42 or Ras GTPases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of RhoGEF p115 of human origin and Lsc of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 748-912 mapping at the C-terminus of RhoGEF p115 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 748-912 mapping at the C-terminus of RhoGEF p115 of human origin |
| Entrez Gene | 9138 |
|---|
Application
Western blot analysis of RhoGEF p115 expression in non-transfected 293T: (A), human RhoGEF p115 transfected 293T: (B), K-562 (C) and Jurkat (D) whole cell lysates.
Application
Western blot analysis of RhoGEF p115 expression in non-transfected 293T: (A), human RhoGEF p115 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of RhoGEF p115 expression in non-transfected: (A) and human RhoGEF p115 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RhoGEF p115 expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane and cytoplasmic staining of cells in white and red pulps at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of RhoGEF p115 expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of RhoGEF p115 of human origin and Lsc of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
