Application
Western blot analysis of PCNP expression in HeLa (A), K-562 (B), Jurkat (C) and Hep G2 (D) nuclear extracts and Jurkat whole cell lysate (E).Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of PCNP expression in HeLa (A), K-562 (B), Jurkat (C) and Hep G2 (D) nuclear extracts.Application
Western blot analysis of PCNP expression in HeLa whole cell lysate.Application
Western blot analysis of PCNP expression in non-transfected: (A) and human PCNP transfected: (B) 293T whole cell lysates.| Product Name | PCNP Antibody (K-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of PCNP of human origin |
| Modification Notes | PCNP (PEST proteolytic signal containing nuclear protein) is a novel 178 amino acid nuclear protein implied to play a role in cell cycle regulation and tumorigenesis. PCNP is ubiquitinated post-translationally by NIRF (Np95/ICBP90-like RING finger protein), a ubiquitin ligase. Existing as three isoforms produced by alternative splicing events, PCNP is encoded by a gene mapping to human chromosome 3q12.3. Chromosome 3 houses over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Key tumor suppressing genes on chromosome 3 include those that encode the apoptosis mediator RASSF1, the cell migration regulator HYAL1 and the angiogenesis suppressor SEMA3B. Marfan Syndrome, porphyria, von Hippel-Lindau syndrome, osteogenesis imperfecta and Charcot-Marie-Tooth Disease are a few of the numerous genetic diseases associated with chromosome 3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PCNP of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with isoform PCNP2 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of PCNP of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of PCNP of human origin |
| Entrez Gene | 57092 |
|---|
Application
Western blot analysis of PCNP expression in HeLa (A), K-562 (B), Jurkat (C) and Hep G2 (D) nuclear extracts and Jurkat whole cell lysate (E).
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of PCNP expression in HeLa (A), K-562 (B), Jurkat (C) and Hep G2 (D) nuclear extracts.
Application
Western blot analysis of PCNP expression in HeLa whole cell lysate.
Application
Western blot analysis of PCNP expression in non-transfected: (A) and human PCNP transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of PCNP of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with isoform PCNP2 also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
