Application
Western blot analysis of p22-phox expression in non-transfected 293T: (A), human p22-phox transfected 293T: (B) and Raw 264.7 (C) whole cell lysates.Application
Western blot analysis of p22-phox expression in non-transfected: (A) and human p22-phox transfected: (B) 293T whole cell lysates.Application
Western blot analysis of p22-phox expression in non-transfected 293T: (A), mouse p22-phox transfected 293T: (B) and Raw 264.7 (C) whole cell lysates.Application
Western blot analysis of p22-phox expression in non-transfected 293T: (A), human p22-phox transfected 293T: (B) and Raw 264.7 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of p22-phox expression in THP-1 (A) and Raw 264.7 (B) whole cell lysates.| Product Name | p22-phox Antibody (FL-195) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 1-195 representing full length p22-phox of human origin |
| Modification Notes | Mox1 and the glycoprotein gp91-phox are largely related proteins that are essential components of the NADPH oxidase. The superoxide-generating NADPH oxidase is present in phagocytes, neuroepithelial bodies, vascular smooth muscle cells and endothelial cells. It includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane, where they associate with the flavocytochrome cytochrome b558 to form the active enzyme complex. The p22- and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p22-phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 1-195 representing full length p22-phox of human origin |
|---|---|
| Tissue Specificity | raised against amino acids 1-195 representing full length p22-phox of human origin |
| Entrez Gene | 1535 |
|---|
Application
Western blot analysis of p22-phox expression in non-transfected 293T: (A), human p22-phox transfected 293T: (B) and Raw 264.7 (C) whole cell lysates.
Application
Western blot analysis of p22-phox expression in non-transfected: (A) and human p22-phox transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of p22-phox expression in non-transfected 293T: (A), mouse p22-phox transfected 293T: (B) and Raw 264.7 (C) whole cell lysates.
Application
Western blot analysis of p22-phox expression in non-transfected 293T: (A), human p22-phox transfected 293T: (B) and Raw 264.7 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of p22-phox expression in THP-1 (A) and Raw 264.7 (B) whole cell lysates.| Application Notes | recommended for detection of p22-phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
