Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells.Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293 whole cell lysates.Application
Western blot analysis of LXRα expression in non-transfected: (A) and mouse LXRα transfected: (B) 293T whole cell lysates.Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293T whole cell lysates.Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293T whole cell lysates.| Product Name | LXRα/β Antibody (S-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of LXRβ of human origin |
| Modification Notes | Retinoids are metabolites of vitamin A (retinol) and are believed to represent important signaling molecules during vertebrate development and tissue differentiation. The cooperation of liver X receptors (LXRs) å and ∫ and retinoic X receptor (RXR) modulate the expression of several genes involved in lipid metabolism in hepatocyte and macrophages. RXR is the receptor for 9-cis retinoic acid and dimerizes with VDR, TR, PPAR and several novel receptors including liver X receptors LXRå (also referred to as RLD-1), LXR∫ and FXR. FXR and LXR fall into a category of proteins termed “orphan receptors” because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. LXRå and LXR∫ share considerable sequence homology and several functions, respond to the same endogenous and synthetic ligands and play critical roles in maintaining lipid homeostasis. LXR∫ is ubiquitously expressed and enriched in tissues of neuronal and endocrine origin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of LXRα and LXRβ of mouse, rat, human, avian and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of LXR&beta of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of LXRβ of human origin |
| Entrez Gene | 7376 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing nuclear and cytoplasmic staining of glandular cells.
Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of LXRα expression in non-transfected: (A) and mouse LXRα transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of LXRβ expression in non-transfected: (A) and human LXRβ transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of LXRα and LXRβ of mouse, rat, human, avian and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
