Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cell projection localization.Application
Western blot analysis of Cdc42 expression in non-transfected 293T: (A), mouse Cdc42 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Cdc42 expression in non-transfected 293T: (A), human Cdc42 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Cdc42 expression in non-transfected control (A) and Cdc42 siRNA transfected (B) NIH/3T3 cells. Blot probed with Cdc42 (P1): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of Cdc42 expression in Jurkat (A), 3611-RF (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.| Product Name | Cdc42 Antibody (P1) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Cdc42 of human origin |
| Modification Notes | The superfamily of GTP-binding proteins, for which the Ras proteins are prototypes, has been implicated in regulation of diverse biological activities involving various aspects of cell growth and division. One mammalian member of the family, Cdc42, has an amino acid sequence that is similar to those of various members of the Ras superfamily proteins, including N-, K- and H-Ras, Rho proteins and the Rac proteins. On the basis of in vitro phosphorylation studies, it has been suggested that human Cdc42 may function in the signaling pathway of the EGF receptor or related growth factor receptor protein kinases. The Dbl oncogene has been shown to specifically catalyze dissociation of GDP from human Cdc42. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cdc42 and Rac1 of mouse, rat, human, Xenopus and Drosophila origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Cdc42 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Cdc42 of human origin |
| Entrez Gene | 998 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane and cell projection localization.
Application
Western blot analysis of Cdc42 expression in non-transfected 293T: (A), mouse Cdc42 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Cdc42 expression in non-transfected 293T: (A), human Cdc42 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Cdc42 expression in non-transfected control (A) and Cdc42 siRNA transfected (B) NIH/3T3 cells. Blot probed with Cdc42 (P1): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of Cdc42 expression in Jurkat (A), 3611-RF (B), HeLa (C) and NIH/3T3 (D) whole cell lysates.| Application Notes | recommended for detection of Cdc42 and Rac1 of mouse, rat, human, Xenopus and Drosophila origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
