Application
Western blot analysis of BHMT expression in human fetal liver tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.Application
Western blot analysis of BHMT expression in non-transfected: (A) and mouse BHMT transfected: (B) 293T whole cell lysates.| Product Name | BHMT Antibody (O-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of BHMT of mouse and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NF˚B, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of BHMT of mouse and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of BHMT of mouse and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 635 |
|---|
Application
Western blot analysis of BHMT expression in human fetal liver tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.
Application
Western blot analysis of BHMT expression in non-transfected: (A) and mouse BHMT transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
