Application
Western blot analysis of Cardiotrophin-1 expression in Hep G2 whole cell lysate.| Product Name | Cardiotrophin-1 Antibody (W-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Cardiotrophin-1 of human origin by WB, IP and ELISA |
| Modification Notes | Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and has been shown to induce hypertrophy in cardiac myocytes in vitro. Cardiotrophin-1, a secreted protein expressed at high levels in myocardium during cardiogenesis, has been shown to promote proliferation and survival of embryonic cardiomyocytes, suggesting a role for Cardiotrophin-1 in the activation of gp130 during cardiac development. Cardiotrophin-1 is highly expressed in heart, prostate, ovary and skeletal muscle. Lower levels of expression are seen in lung, kidney, pancreas, thymus, testis and small intestine. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Cardiotrophin-1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Cardiotrophin-1 of human origin by WB, IP and ELISA |
| Entrez Gene | 1489 |
|---|
Application
Western blot analysis of Cardiotrophin-1 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
