Application
Western blot analysis of PCYOX1 expression in non-transfected 293T: (A), human PCYOX1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.Application
Western blot analysis of PCYOX1 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing cytoplasmic localization.| Product Name | PCYOX1 Antibody (E-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PCYOX1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | PCYOX1 (prenylcysteine oxidase 1), also known as prenylcysteine lyase or PCL1, is a 505 amino acid FAD-dependent thioether oxidase belonging to the prenylcysteine oxidase family. Ubiquitously expressed, PCYOX1 has been found to catalyze the degradation of prenylcysteine into free cysteines and a hydrophobic isoprenoid, and is released during the degradation of prenylated proteins. PCYOX1 specifically cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. PCYOX1 is glycosylated at multiple N-glycosylation sites and is encoded by a gene that maps to human chromosome 2p14. PCYOX1 has recently been implied to play a significant role in atherogenesis, and may function as a VLDL (very low-density lipoprotein)-associated protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PCYOX1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PCYOX1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 51449 |
|---|
Application
Western blot analysis of PCYOX1 expression in non-transfected 293T: (A), human PCYOX1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.
Application
Western blot analysis of PCYOX1 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
