Application
Western blot analysis of CHAD expression in 293T whole cell lysate.Application
Western blot analysis of CHAD expression in non-transfected: (A) and mouse CHAD transfected: (B) 293T whole cell lysates.| Product Name | CHAD Antibody (Y-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CHAD of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | CHAD, also known as chondroadherin, cartilage leucine-rich protein or SLRR4A, is a 359 amino acid cartilage matrix protein that plays a significant role in the regulation of chondrocyte growth and proliferation. CHAD is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin å2/∫1. Existing primarily in monomeric form, CHAD is a secreted protein that localizes to the extracellular matrix and belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily. CHAD contains 11 LRR (leucine-rich) repeats and is present in chondrocytes of all ages. The gene encoding CHAD maps to human chromosome 17q21.33 and mouse chromosome 11 D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CHAD of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of CHAD of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 1101 |
|---|
Application
Western blot analysis of CHAD expression in 293T whole cell lysate.
Application
Western blot analysis of CHAD expression in non-transfected: (A) and mouse CHAD transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
