Application
Western blot analysis of Sm B/B'/N expression in K-562 (A), HeLa (B) and KNRK (C) nuclear extracts.Application
Western blot analysis of Sm B/B'/N expression in HeLa (A), KNRK (B) and K-562 (C) nuclear extracts.Application
Western blot analysis of Sm B/B'/N expression in non-transfected: (A) and human Sm B/B'/N transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of Sm N expression in non-transfected: (A) and mouse Sm N transfected: (B) 293T whole cell lysates and K-562 nuclear extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Sm B expression in non-transfected control (A) and Sm B siRNA transfected (B) HeLa cells. Blot probed with Sm B/B'/N (FL-240): . GAPDH (FL-335): used as specificity and loading control.| Product Name | Sm B/B'/N Antibody (FL-240) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-240 representing full length Sm B of human origin |
| Modification Notes | mRNA precursors are processed in the spliceosome, where introns are excised to form continuous coding sequences. The major components of the spliceosome are RNA-protein complexes called snRNPs (small nuclear ribonucleoprotein particles) (1). The core proteins that are common to all snRNPs are called the Sm proteins, and are designated B, B’, D1, D2, D3, E, F and G (2). Antibodies recognizing Sm proteins are frequently generated in autoimmune diseases, including in patients with systemic lupus erythematosus (3). Sm proteins are characterized by a conserved Sm sequence motif in two parts, Sm1 and Sm2, which are separated by a variable region (4, 5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Sm B, Sm B' and Sm N of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-240 representing full length Sm B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-240 representing full length Sm B of human origin |
| Entrez Gene | 6628 |
|---|
Application
Western blot analysis of Sm B/B'/N expression in K-562 (A), HeLa (B) and KNRK (C) nuclear extracts.
Application
Western blot analysis of Sm B/B'/N expression in HeLa (A), KNRK (B) and K-562 (C) nuclear extracts.
Application
Western blot analysis of Sm B/B'/N expression in non-transfected: (A) and human Sm B/B'/N transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of Sm N expression in non-transfected: (A) and mouse Sm N transfected: (B) 293T whole cell lysates and K-562 nuclear extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing nuclear staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Sm B expression in non-transfected control (A) and Sm B siRNA transfected (B) HeLa cells. Blot probed with Sm B/B'/N (FL-240): . GAPDH (FL-335): used as specificity and loading control.| Application Notes | recommended for detection of Sm B, Sm B' and Sm N of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
