Application
Western blot analysis of rat recombinant Rho B.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of Rho B expression in non-transfected: (A) and mouse Rho B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rho B expression in non-transfected 293T: (A), mouse Rho B transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of Rho B expression in non-transfected control (A) and Rho B siRNA transfected (B) NIH/3T3 cells. Blot probed with Rho B (119): . β Tubulin (D-10): used as specificity and loading control.Application
Western blot analysis of Rho B expression in lysates from UV-treated HeLa cells.| Product Name | Rho B Antibody (119) PE |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping to an internal region of Rho B of human origin |
| Modification Notes | The Ras p21 family of guanine nucleotide proteins has been widely studied in view of its apparent role in signal transduction pathways and high frequency of mutations in human malignancies. It is now clear, however, that the Ras proteins (H-, K- and N-Ras p21) are members of a much larger superfamily of related proteins. Six members of this family, Rap 1A, Rap 1B, Rap 2, R-Ras, Ral A and Ral B, exhibit approximately 50% amino acid homology to Ras. The six mammalian Rho proteins (Rho A, B, C, G, 7 and 8) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy-terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Rho B p21 and, to a lesser extent, Rho A of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping to an internal region of Rho B of human origin |
|---|---|
| Tissue Specificity | epitope mapping to an internal region of Rho B of human origin |
| Entrez Gene | 388 |
|---|
Application
Western blot analysis of rat recombinant Rho B.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of Rho B expression in non-transfected: (A) and mouse Rho B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rho B expression in non-transfected 293T: (A), mouse Rho B transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of Rho B expression in non-transfected control (A) and Rho B siRNA transfected (B) NIH/3T3 cells. Blot probed with Rho B (119): . β Tubulin (D-10): used as specificity and loading control.
Application
Western blot analysis of Rho B expression in lysates from UV-treated HeLa cells.| Application Notes | recommended for detection of Rho B p21 and, to a lesser extent, Rho A of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
