Application
Western blot analysis of p55 CDC expression in non-transfected 293T: (A), human p55 CDC transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of p55 CDC expression in non-transfected 293T: (A), mouse p55 CDC transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of p55 CDC expression in non-transfected control (A) and p55 CDC siRNA transfected (B) HeLa cells. Blot probed with p55 CDC (H-175): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of p55 CDC expression in K-562 (A), MOLT-4 (B), Ramos (C) and U-937 (D) whole cell lysates.| Product Name | p55 CDC Antibody (H-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-175 of p55 CDC of human origin |
| Modification Notes | Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13suc 1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase termed p55 CDC exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55 CDC transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested. p55 CDC shows kinase activity towards a-casein and myelin basic protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p55 CDC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-175 of p55 CDC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-175 of p55 CDC of human origin |
| Entrez Gene | 991 |
|---|
Application
Western blot analysis of p55 CDC expression in non-transfected 293T: (A), human p55 CDC transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of p55 CDC expression in non-transfected 293T: (A), mouse p55 CDC transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of p55 CDC expression in non-transfected control (A) and p55 CDC siRNA transfected (B) HeLa cells. Blot probed with p55 CDC (H-175): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of p55 CDC expression in K-562 (A), MOLT-4 (B), Ramos (C) and U-937 (D) whole cell lysates.| Application Notes | recommended for detection of p55 CDC of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
