Application
Western blot analysis of Cdc37 expression in non-transfected: (A) and human Cdc37 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Cdc37 expression in non-transfected 293T: (A), human Cdc37 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Cdc37 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Cdc37 (H-271): .Application
Western blot analysis of Cdc37 expression in A-431 (A), Jurkat (B), CTLL-2 (C), K-562 (D) and LNCaP (E) whole cell lysates and rat testis (F) tissue extract.Application
Western blot analysis of human recombinant Cdc37 fusion protein.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining (A). Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization (B).| Product Name | Cdc37 Antibody (H-271) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 108-378 of Cdc37 of human origin |
| Modification Notes | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cdc37 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 108-378 of Cdc37 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 108-378 of Cdc37 of human origin |
| Entrez Gene | 11140 |
|---|
Application
Western blot analysis of Cdc37 expression in non-transfected: (A) and human Cdc37 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Cdc37 expression in non-transfected 293T: (A), human Cdc37 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Cdc37 siRNA silenced HeLa (B) cells showing diminished nuclear staining in the siRNA silenced cells. Cells probed with Cdc37 (H-271): .
Application
Western blot analysis of Cdc37 expression in A-431 (A), Jurkat (B), CTLL-2 (C), K-562 (D) and LNCaP (E) whole cell lysates and rat testis (F) tissue extract.
Application
Western blot analysis of human recombinant Cdc37 fusion protein.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining (A). Immunofluorescence staining of methanol-fixed KNRK cells showing cytoplasmic localization (B).| Application Notes | recommended for detection of Cdc37 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
