Application
Western blot analysis of human recombinant MMP-2.Application
Immunofluorescence staining of normal mouse intestine frozen section showing extracellular staining.Application
Western blot analysis of MMP-2 expression in ECV304 (A) and A-375 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-375 cells showing cytoplasmic localization.| Product Name | MMP-2 Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-76 mapping at the N-terminus of MMP-2 of human origin |
| Modification Notes | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MMP-2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-76 mapping at the N-terminus of MMP-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-76 mapping at the N-terminus of MMP-2 of human origin |
| Entrez Gene | 4313 |
|---|
Application
Western blot analysis of human recombinant MMP-2.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing extracellular staining.
Application
Western blot analysis of MMP-2 expression in ECV304 (A) and A-375 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-375 cells showing cytoplasmic localization.| Application Notes | recommended for detection of MMP-2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
