Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.Application
Western blot analysis of TACE expression in non-transfected control (A) and TACE siRNA transfected (B) HeLa cells. Blot probed with TACE (H-300): . α-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of TACE expression in NAMALWA (A) and K-562 (B) whole cell lysates.| Product Name | TACE Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the amino terminus of TACE (also designated ADAM17) of human origin |
| Modification Notes | Tumor necrosis factor ∫ (TNF∫), also known as lymphotoxin, is a pleiotropic cytokine. TNFå, also known as cachetin, is a cytokine that binds to the same receptors, producing an array of effects similar to those of TNF∫. TNF∫ and TNFå share 30% amino acid homology and have similar biological activities. TNF∫ is produced by activated lymphocytes, including CD4(+) T helper cell type 1 lymphocytes, CD8(+) lymphocytes and certain B lymphoblastoid cell lines. TNFå is produced by several different cell types, including lymphocytes, neutrophils and macrophages. TNF∫ and TNFå can modulate many immune and inflammatory functions while having the ability to inhibit tumor growth. TACE (for TNFå converting enzyme) is a metalloproteinase that cleaves the membrane-bound TNFå precursor to release soluble TNFå. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of TACE precursor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the amino terminus of TACE (also designated ADAM17) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the amino terminus of TACE (also designated ADAM17) of human origin |
| Entrez Gene | 6868 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human brain tissue showing cytoplasmic staining of neuronal cells.
Application
Western blot analysis of TACE expression in non-transfected control (A) and TACE siRNA transfected (B) HeLa cells. Blot probed with TACE (H-300): . α-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of TACE expression in NAMALWA (A) and K-562 (B) whole cell lysates.| Application Notes | recommended for detection of TACE precursor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
