Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of ADAMTS-1 expression in KNRK whole cell lysate.Application
Western blot analysis of mature ADAMTS-1 expression in KNRK whole cell lysate.| Product Name | ADAMTS-1 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 806-865 mapping near the C-terminus of ADAMTS-1 of human origin |
| Modification Notes | ADAMTS-1, also known as METH-1, C3-C5 and KIAA1346, and the related protein ADAMTS-8, also known as METH-2 and FLJ41712, represent a new family of proteins with metalloprotease, disintegrin and Thrombospondin domains. ADAMTS-1 and ADAMTS-2 are secreted and proteolytically processed proteins that are 51.7% identical but display different, non-overlapping patterns of expression in tissues and cultured celllines. Both ADAMTS proteins have been shown to be more active than Thrombospondin-1 or endostatin in preventing angiogenesis in a cornea pocket model, and both may have application for the inhibition of new blood vessel formation in a range of tumor types. The spacer region and the Thrombospondin type I motifs in the carboxy-terminus of ADAMTS-1 are important for anchoring ADAMTS-1 to the extracellular matrix. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ADAMTS-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 806-865 mapping near the C-terminus of ADAMTS-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 806-865 mapping near the C-terminus of ADAMTS-1 of human origin |
| Entrez Gene | 9510 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of ADAMTS-1 expression in KNRK whole cell lysate.
Application
Western blot analysis of mature ADAMTS-1 expression in KNRK whole cell lysate.| Application Notes | recommended for detection of ADAMTS-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
